Born in Berlin, the mezzo-soprano Stepanka Pucalkova graduated in 2005 studying singing at the High school of Jan Neruda in Prague and 2007 she continued her studies at the University Mozarteum in Salzburg, Austria. There she worked under the guidance of Elisabeth Wilke, Josef Wallnig and Eike Gramss and in the year of 2012 she received her master's degree in opera. The same year Stepanka Pucalkova was awarded by the organization Stiftung the Mozarteum Medal of Lilli Lehmann. 

She performed in a number of productions at the Salzburg Mozarteum University and Landestheater in Salzburg, including roles such as Mozart´s Idamante (Idomeneo), 2nd Lady (The Magic Flute) and Marcellina (The Marriage of Figaro), Octavian (R. Strauss: Der Rosenkavalier), Larina ( Tchaikovsky: Eugene Onegin), Galatea (Handel: Aci, Galatea et Polifemo) and Annina (Verdi: La traviata). In summer of  2013 she performed Lehrbube/Die Meistersinger von Nürnberg at the Haus for Mozart (Salzburger Festspiele) with conductor Danielle Gatti and stage director Stefan Herheim. In March 2014 in Salzburg she presented herself as Sesto (Mozart: La clemenza di Tito) and in the same role in Summer of 2014 at Teatro Romano Fiesole in Florenz (Italy) 

Worked with famous conductors and directors as Danielle Gatti, Stefan Herheim, Mariusz Trélinski, Mirga Granžynite-Tyla, Friedrich Haider, Marco Guidarini, Jiří Heřman, Leo Hussain, Eike Gramss, Keith Warner and Roland Böer. 

In Autumn 2014 Stepanka Pucalkova performed as a guest in the Prague State Opera in the role of the Servant (R. Strauss: Salome), in the Slovak National Theatre in Bratislava (Rosina in Rossini's Barber of Seville and Annius in La clemenza di Tito), as well as Mercedes (Bizet: Carmen) in Landestheater Bregenz und Landesthetare Salzburg. 

In season 15/16 the young mezzo made her role debut as Suzuki (G. Puccini: Madama Butterfly), also performed the roles of Stéphano (Ch. Gounod: Roméo et Juliett), 3rd Dame (W.A.Moazrt: The magic flute) and 1. Maidservant (R. Strauss: Elektra) in the State opera Prague. In Autumn 2016 she performed the role of 3rd Lady in „Die Zauberflöte“ on japanese tour with Prague State opera.

In season 16/17 Stepanka performed El Sombrero de tres picos by Manuel De Falla in Kristianand, Siegrune/Die Walküre with Christian Thieleman at Osterfestspiele Salzburg and at Beijing Music Festival, also Suzuki/Madama Butterfly at State Opera Prague and 3rd Lady/The Magic Flute on tour in Japan with Prague State Opera.

Most recently Stepanka Pucalkova has performed Charlotte/Werther in State Opera Prague and El Sombrero de tres picos by De Fallain with Kristiansand Symfoniorkester.

Future engagements include Christmas Oratiorio with Copenahagen Royal Chapel Choir, Mozart's Davide Penitente at Mozarteum Salzburg as well as Charlotte/Werther, Stéphano/Roméo et Juliette and Suzuki/Madama Butterfly in State Opera Prague. Stepanka Pucalkova is also engaged in ensemble with Semperoper Dresden where in season 2018/2019 she will perform among others 2nd Solostimme/Moses und Aron, Mercédès/Carmen, Cherubino/Figaro and Urbain/Hugenotten.

In December 2016 she won the second prize in category "The best female voice" on Concours international de Belcanto Vincenzo Bellini" in Marseille.

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“Štěpánka Pučálková presented herself very nicely as her (Cio Cio Sans) devoted companion and maid Suzuki, her darkly coloured mezzo stood out especially in the last act before the suicide of Cio-Cio-San."

“Among other soloists, Štěpánka Pučálková beautifully expressed empathy towards Cio-Cio-San with her distinctive mezzo-soprano.”

“ well as Štěpánka Pučálková, who coped with the role of Suzuki with solid technique and acting credibility”

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